NVITe Lab — Research and Publications

 

Laser Speckle of I/R

RESEARCH

Our lab investigates the interactions among innate immune cells, cerebrovascular blood flow, and behavior in preclinical models of stroke and in human stroke. For the preclinical work, the lab employs various technologies to achieve this goal, including confocal microscopy, light-sheet microscopy, deep-learning algorithms, live-animal imaging, immunologic manipulation with flow cytometry readouts, transgenic mouse models, and advanced surgical techniques. For the human work, we study behavioral data and link it to immune changes we detect in the blood of stroke patients. We believe that understanding these relationships and spatiotemporal differences can lead to more precise therapies for innate immunity in stroke, with meaningful improvements for patients.

 

Advanced confocal microscopy of inflammatory histopathologic components in stroke

 

IMARIS

Advanced confocal microscopy of inflammatory histopathologic components in stroke, using IMARIS.

 

DTI of a Stroke

Diffusion Tensor Imaging of a Stroke Mouse Brain

 

DeepLabCut of a Mouse in an Open Field Test

DeepLabCut of a Mouse in an Open-Field Test

SEM of a Human Stroke Clot

Scanning Electron Microscopy of a Human Stroke Clot

 

PUBLICATIONS

Arias E, Haynes ME, Nadkarni NA, Lipfert ZK, Muller WA, Batra A, Sullivan DP. J Cell Sci. 2025 Apr 15;138(8). doi: 10.1242/jcs.263835. Epub 2025 Apr 22. PubMed PMID: 40260638; PubMed Central PMCID: PMC12136169.

Madeira MM, Hage Z, Kokkosis AG, Nnah K, Guzman R, Schappell LE, Koliatsis D, Resutov E, Nadkarni NA, Rahme GJ, Tsirka SE. Glia. 2025 Jun;73(6):1130-1147. doi: 10.1002/glia.24661. Epub 2024 Dec 24. PubMed PMID: 39719686; PubMed Central PMCID: PMC12014386.

Dammavalam V, Rupert D, Lanio M, Jin Z, Nadkarni N, Tsirka SE, Bergese SD. Int J Mol Sci. 2024 Jul 16;25(14). doi: 10.3390/ijms25147772. Review. PubMed PMID: 39063013; PubMed Central PMCID: PMC11276729.

Li Y, Schappell LE, Polizu C, DiPersio J, Tsirka SE, Halterman MW, Nadkarni NA. J Clin Med. 2023 Oct 24;12(21). doi: 10.3390/jcm12216715. Review. PubMed PMID: 37959180; PubMed Central PMCID: PMC10649331.

Nadkarni NA, Arias E, Fang R, Haynes ME, Zhang HF, Muller WA, Batra A, Sullivan DP. Am J Pathol. 2022 Nov;192(11):1619-1632. doi: 10.1016/j.ajpath.2022.07.008. Epub 2022 Aug 8. PubMed PMID: 35952762; PubMed Central PMCID: PMC9667712.

Nadkarni NA, Maas MB, Batra A, Kim M, Manno EM, Sorond FA, Prabhakaran S, Naidech AM, Liotta EM. J Stroke Cerebrovasc Dis. 2020 Apr;29(4):104605. doi: 10.1016/j.jstrokecerebrovasdis.2019.104605. Epub 2020 Jan 11. PubMed PMID: 31932209; PubMed Central PMCID: PMC7066873.

Beckmann L, Zhang X, Nadkarni NA, Cai Z, Batra A, Sullivan DP, Muller WA, Sun C, Kuranov R, Zhang HF. Biomed Opt Express. 2019 Oct 1;10(10):5235-5250. doi: 10.1364/BOE.10.005235. eCollection 2019 Oct 1. PubMed PMID: 31646044; PubMed Central PMCID: PMC6788609.

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